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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
Fecha : |
15/07/2015 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Revista INIA |
Autor : |
INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA) |
Título : |
Revista INIA Uruguay. (No.7, Junio 2006). |
Fecha de publicación : |
2006 |
Fuente / Imprenta : |
Montevideo (Uruguay): INIA, 2006. |
Páginas : |
48 p. |
Serie : |
(Revista INIA; 07) |
ISSN : |
1510-9011 |
Idioma : |
Español |
Thesagro : |
ARROZ; BIOTECNOLOGIA; BOVINOS DE CARNE; CAMBIO CLIMÁTICO; CIENCIA; CITRUS; CLIMA; CLIMATOLOGIA; COMUNICACIÓN; CONTROL DE ENFERMEDADES; CULTIVOS DE GRANO; CULTIVOS DE SECANO; ENTOMOLOGIA; ESPECIES FORRAJERAS; EUCALYPTUS; EXPLOTACION AGRICOLA FAMILIAR; FITOPATOLOGÍA; FORESTALES; FORRAJES; FRUTALES; FRUTICULTURA; GANADO BOVINO; GRANOS; GRAS; HORTALIZAS; HORTICULTURA; INIA; INNOVACION; INVESTIGACIÓN; LECHERÍA; LEGUMINOSAS FORRAJERAS; MANEJO DEL CULTIVO; MEJORAMIENTO ANIMAL; METEOROLOGIA; MICROBIOLOGÍA; OVINOS; PASTURAS; PRODUCCIÓN ANIMAL; PRODUCCION DE LANA; PRODUCCION DE LECHE; PRODUCCION LECHERA; REVISTA INIA 2006; SEMILLAS; SOJA; SUELOS; SUINOS; SUSTENTABILIDAD AMBIENTAL; TECNOLOGÍA; TRANSFERENCIA DE TECNOLOGIA; VARIEDADES; VITICULTURA. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/4816/1/Revista-INIA-07.pdf
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Marc : |
LEADER 01904nam a2200745 a 4500 001 1053069 005 2019-10-15 008 2006 bl uuuu u00u1 u #d 022 $a1510-9011 100 1 $aINIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA) 245 $aRevista INIA Uruguay. (No.7, Junio 2006). 260 $aMontevideo (Uruguay): INIA$c2006 300 $a48 p. 490 $a(Revista INIA; 07) 650 $aARROZ 650 $aBIOTECNOLOGIA 650 $aBOVINOS DE CARNE 650 $aCAMBIO CLIMÁTICO 650 $aCIENCIA 650 $aCITRUS 650 $aCLIMA 650 $aCLIMATOLOGIA 650 $aCOMUNICACIÓN 650 $aCONTROL DE ENFERMEDADES 650 $aCULTIVOS DE GRANO 650 $aCULTIVOS DE SECANO 650 $aENTOMOLOGIA 650 $aESPECIES FORRAJERAS 650 $aEUCALYPTUS 650 $aEXPLOTACION AGRICOLA FAMILIAR 650 $aFITOPATOLOGÍA 650 $aFORESTALES 650 $aFORRAJES 650 $aFRUTALES 650 $aFRUTICULTURA 650 $aGANADO BOVINO 650 $aGRANOS 650 $aGRAS 650 $aHORTALIZAS 650 $aHORTICULTURA 650 $aINIA 650 $aINNOVACION 650 $aINVESTIGACIÓN 650 $aLECHERÍA 650 $aLEGUMINOSAS FORRAJERAS 650 $aMANEJO DEL CULTIVO 650 $aMEJORAMIENTO ANIMAL 650 $aMETEOROLOGIA 650 $aMICROBIOLOGÍA 650 $aOVINOS 650 $aPASTURAS 650 $aPRODUCCIÓN ANIMAL 650 $aPRODUCCION DE LANA 650 $aPRODUCCION DE LECHE 650 $aPRODUCCION LECHERA 650 $aREVISTA INIA 2006 650 $aSEMILLAS 650 $aSOJA 650 $aSUELOS 650 $aSUINOS 650 $aSUSTENTABILIDAD AMBIENTAL 650 $aTECNOLOGÍA 650 $aTRANSFERENCIA DE TECNOLOGIA 650 $aVARIEDADES 650 $aVITICULTURA
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
05/04/2022 |
Actualizado : |
01/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
Páginas : |
13 p. |
DOI : |
10.3389/fpls.2022.804839 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
Asunto categoría : |
H10 Plagas de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
LEADER 02536naa a2200277 a 4500 001 1062960 005 2022-09-01 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fpls.2022.804839$2DOI 100 1 $aSCHVARTZMAN, C. 245 $aRNAi in Piezodorus guildinii (Hemiptera$bPentatomidae): transcriptome assembly for the development of pest control strategies.$h[electronic resource] 260 $c2022 300 $a13 p. 500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario. 520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. 653 $aDSRNA, 653 $aPEST CONTROL 653 $aRNA-SEQ 653 $aRNAI 653 $aSTINK BUG 653 $aVATPASE A, 700 1 $aFRESIA, P. 700 1 $aMURCHIO, S. 700 1 $aMUJICA, V. 700 1 $aDALLA RIZZA, M. 773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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